Hepatoprotective Effects of Calotropis procera (Ait.) R. Br Root Bark Extracts against Diethylnitrosamine Induced Liver Injury in Rats

Background: Diethylnitrosamine (DEN) is a hepatotoxin whose metabolic activation by liver cytochromes P450 responsible for the necrosis, mutagenicity and carcinogenicity of cells. The purpose this study was to evaluate protective effects Calotropis procera roots bark against DEN induced hepatocellular damage in rats.
 Material Methods: Hepatoprotective activity ethanolic extract root were evaluated induction injury with Wistar male rats distributed six groups six. Serum hepatic markers, alanine amino transferase (ALAT), aspartate (ASAT), alkaline phosphatase (ALP), total protein albumin enzymes antioxidant activities, superoxide dismutase (SOD) catalase, as well level malonedialdehyde (MDA) determined homogenate. Histological analysis carried out on sections rat livers. Phytoconstituents have also been studied. Results: Pretreatment showed significant decrease ALAT, ASAT ALP while there an increase compared treated only DEN. It SOD catalase MDA levels suggesting hepatoprotective effect extract. Observation confirmed results biochemical parameters which would attest that hepatoprotective. such sterols, triterpenes phenolic compounds demonstrated.
 Conclusion: Ethanolic has shown could be due its content sterols triterpenic compounds.